Nature of Melittin-Phospholipid Interaction
نویسندگان
چکیده
منابع مشابه
Calorimetric Determination of Phospholipid- Melittin Interactions
The effect of melittin incorporated into the phospholipid bilayer of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at mole fractions up to 3.75 mol% have been investigated. The study reveals a considerable influence of melittin on the phase transition profile and volumetric properties of the DPPC bilayer. The temperature dependence of the coefficient of expansion, , and the volume fluctua...
متن کاملInteraction of melittin with endotoxic lipid A.
Several amphipathic and cationic substances are known to bind lipid A, the toxic component of bacterial lipopolysaccharides. In this report, we have characterized, by fluorescence methods, the interaction of melittin, an amphipathic and basic 26-residue polypeptide isolated from bee venom, with lipid A. The stoichiometry of the complex appears to be two molecules of melittin to one of lipid A w...
متن کاملInteraction of bee venom melittin with zwitterionic and negatively charged phospholipid bilayers: a spin-label electron spin resonance study.
Electron spin resonance (ESR) spectroscopy was used to study the penetration and interaction of bee venom melittin with dimyristoylphosphatidylcholine (DMPC) and ditetradecylphosphatidylglycerol (DTPG) bilayer membranes. Melittin is a surface-active, amphipathic peptide and serves as a useful model for a variety of membrane interactions, including those of presequences and signal peptides, as w...
متن کاملMolecular dynamics simulation of interaction of Melittin and DMPC bilayer: Temperature dependence
The interaction between proteins and membranes has an important role in biological pro-cesses.We have calculated energies of interaction between Melittin and DMPC bilayer in differenttemperatures. We have used the CHARMM software for MD simulation under the canonical (N,V, E) ensemble at different temperatures. The computations have shown that water moleculeshave more penetration into the bilay...
متن کاملInteraction of alytic polypeptide, melittin, with lipid membrane systems.
To study mechanisms whereby lytic proteins disrupt biomembranes, artificial phospholipid spherules (liposomes) were exposed to melittin, a cationic peptide in which sequences of hydrophobic (Positions 1 to 20) and hydrophilic (Positions 21 to 26) amino acids are unequally distributed. At concentrations above 1OF M, melittin, which is the major toxin of bee venom, released marker anions (CrOb) o...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1982
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(82)84649-3